Maintains diagnostic viability of all specimens received. Ensures correct patient labeling of all materials received. Interprets results of special studies, distinguishing negative results from failed studies. Uses independent judgment in tissue fixation issues.
Embeds routine and complex tissue in paraffin, orienting according to tissue type indicated on the worksheet. Checks surgical case number and verifies on a worksheet. Records on grossing sheets the number of blocks received and organize blocks for sectioning.
Sections of paraffin blocks of routine and complex tissue from 2 to 50 microns using a microtome. Mounts paraffin section on microscopic slide and transfers surgical case number to slide. Verifies match of surgical case number between blocks and slides.
Performs frozen sections of routine and complex tissue for immunohistochemistry and enzyme histochemistry.
May perform other specialized histologic procedures such as nerve teasing, limited routine immunohistochemistry and microtomy utilizing a sliding microtome.
Performs H&E staining, and routine and complex special staining of paraffin embedded tissue according to instructions on requisition paperwork. Verifies match of surgical case number between slides and paperwork.
Coverslips prepared slides manually and utilizing automated systems. Records in laboratory logbooks all slides and stains prepared. Logs appropriate information into the surgical pathology computer system. Verifies match of the surgical case number.
Organizes slides for distribution to residents, fellows and/or senior pathologists according to requisition paperwork and worksheets. Documents and follows up with senior pathologist on any errors encountered.
Responsible for filing and accurate record keeping of paraffin tissue blocks.
Responsible for documentation of daily QA/QC charts for all laboratory equipment.
Independently troubleshoot problems relating to routine operation of the laboratory. Evaluate existing protocols and recommends changes.
Orients and trains new and less advanced laboratory staff.
Competent in the use of a pH meter. Understands the theory of molarity and buffer solutions.
Prepares and maintains working solutions for routine H&E staining on automatic stainers, including daily quality control. Prepares and maintains working solutions of routine and complex special stains, including maintaining acceptable working volumes.
Maintains individual microtome and other laboratory equipment in proper working order, following appropriate laboratory safety protocols. Extensive knowledge of all laboratory equipment.
Maintains established department policies, procedures and objectives, including the areas of quality assurance, safety, environmental and infection control.
A.S., B.S. or B.A. in a biological or chemical science and/or completion of a NAACLS-accredited histology technician training program highly desired.
Minimum 2 years experience in Histology required. May take 3+ years of experience and training to be competent to perform work at this level.